Earthworm Freeze Dryer

Earthworm Freeze Dryer

Qhuav earthworms, muaj koob muaj npe jelly, ginseng, vaub kib, vaub kib thiab lwm yam khoom noj khoom haus thiab kev noj qab haus huv, siv cov txheej txheem nqus dej kom qhuav, ntshiab thiab ntuj.

Txoj kev npaj av khov-qhuav hmoov los ntawm lub tshuab nqus tsev vacuum freeze-drying mechanism yog tus cwj pwm uas nws muaj cov kauj ruam hauv qab no:

Kauj Ruam 1: Kev npaj cov hmoov av av ntxhib: cov tsiaj nyob hauv av tau tshaib plab rau ib hnub, cov xuab zeb yog ib txwm nchuav tawm, ntxuav, ces qhuav thiab crushed nyob rau hauv qhov cua qhov cua kub ntawm 2035 degree, thiab earthworm coarse hmoov yog tsim los ntawm 100200 mesh sieve;

Kauj Ruam 2: Kev npaj cov tshuaj enzyme crude: noj cov hmoov av av hmoov av, homogenize nrog 2 npaug ntawm qhov ntim ntawm 0.1mol/l phosphate buffer PBS nrog pH 7.8, ntxiv 0.1mol/lnacl tov, muab tso rau hauv lub tub yees ntawm 4 degree thaum hmo ntuj, ces centrifuge ntawm 10000r / min ntawm 4 degree rau 15min, muab pov tseg los nag thiab coj lub supernatant; Cov supernatant tau sov so hauv dej da dej ntawm 50 degree rau 40 min, txias los ntawm dej ntws, tom qab ntawd centrifuged ntawm 4 degree rau 10 min, coj cov supernatant, ntxiv ammonium sulfate rau supernatant kom ncav cuag 30% saturation, sawv rau 3 teev. , ces centrifuged ntawm 4 degree rau 10 min ntawm 8000 R / min; Sau cov supernatant, ntxiv ammonium sulfate kom ncav cuag 70% saturation, sawv ntsug rau 3 teev, 8000r / min, centrifuge ntawm 4 degree rau 10 min, thiab sau cov nag lossis daus; Dissolve lub precipitate nyob rau hauv lub buffer tov, dialysis lub buffer tov nrog ib tug dialysis hnab uas muaj 5kd molecular hnyav ib hmos kom tshem tawm cov ntsev, ces centrifuge lub dialysate ntawm 8000r / min ntawm 4 degree rau 10min, thiab coj lub supernatant raws li crude earthworm plasmin tov. ;

Kauj Ruam 3: purification ntawm crude enzyme: lub earthworm fibrinolytic enzyme tov tau dhau los ntawm Sephadex G75 gel chromatography, ua ntej kem nrog 0.02mol/L thiab pH raws li qhov tsis txaus ntawm 7.8 phosphate. Tom qab lub kem raug tshem tawm, cov saum toj no tsis tau eluted kom tsis muaj lub teeb haum ntawm 280nm wavelength, thiab elution kua tshwm sim 2 protein peaks, lub ncov ntawm enzyme kev ua si overlapped nrog thawj protein peaks, thiab overlapping ib feem ntawm cov enzyme kua tau sau. Dhau los ntawm deaecellulose a52 ion pauv kem, thiab sib npaug nrog PBS ntawm 0.05mol / l thiab pH 7.8 ua ntej dhau los ntawm kem; Tom qab ntawd nws tau eluted nrog PBS ion zog linear gradient uas muaj 00.5mol/l NaCl. Muaj peb lub ncov protein nyob rau hauv lub eluent, lub enzyme kev ua si ncov overlapped nrog lub thib peb ncov ntawm lub protein ncov, thiab lwm yam ob protein peaks yeej tsis muaj enzyme kev ua si. Cov active ingredient yog earthworm plasmin ntshiab enzyme;

Kauj Ruam 4: Kev hloov kho cov kab mob hauv av plasmin: ntxiv cov kab mob hauv av plasmin ntshiab enzyme thiab activated peg rau 15ml, pH 9.2, 0.1mol / l boric acid tsis. Qhov hnyav piv ntawm ntshiab enzyme rau peg yog 1:50. Khaws qhov kub li 1 teev ntawm 30 degree, thiab cov reactant dialyzes 0.1mol / l, pH 7.3 phosphate tsis nyob rau hmo ntuj. Lub dialysate yog centrifuged ntawm 10000r / min thiab 4 degree rau 10min. Nqa cov supernatant thiab hla hauv kem Sephadex G75, Sau cov khoom nquag, uas yog earthworm plasmin modifying enzyme;

Kauj Ruam 5: Kev npaj cov kab mob hauv av fibrinolytic enzyme lyophilized hmoov: muab earthworm fibrinolytic enzyme hloov kho enzyme nyob rau hauv cov qauv phaj nrog cov khoom thickness tsis ntau tshaj 8 hli, muab tso rau hauv chav ziab ntawm lub tshuab nqus tsev Earthworm Freeze Dryer rau khov-dry, khov nws ntawm - 35 degree rau 12 teev, thiab tom qab ntawd tsa qhov kub thiab txias rau 30 degree hauv qab lub tshuab nqus tsev ntawm 5 Pa thiab qhuav rau 12 teev; Lub freeze-dried hmoov ntawm earthworm plasmin hloov enzyme tau txais los ntawm cua sov rau 40 degree thiab nqus kom qhuav rau 14 teev. Cov ntsiab lus dej yog 7.0%, cov ntsiab lus ntawm cov protein yog 68%, thiab cov enzyme kev ua haujlwm yog 1700u / mg.

Parameter:

Kev muag khoom ua ntej:

* Thaum peb tau txais koj qhov kev nug, peb yuav teb koj tus email raws sijhawm thiab sim peb qhov zoo tshaj plaws los qhia koj lub tshuab ziab khaub ncaws uas haum tshaj plaws. Cov ncauj lus kom ntxaws tech-info yuav muab rau koj.

* Peb tuaj yeem ua cov qauv kuaj rau cov neeg siv khoom.

Tom qab kev muag khoom:

* Yog tias ua tau, thov tuaj rau peb lub Hoobkas thiab peb yuav qhia cov neeg ua haujlwm tag nrho cov txheej txheem ntawm kev ziab kom qhuav. Lub sijhawm tsim nyog tshaj plaws uas yuav tsum tau ua kom khov kom qhuav yuav raug kuaj los ntawm peb.

* Peb yuav tiv tauj koj, kom paub tseeb tias txhua qhov chaw ntawm lub tshuab ziab khaub ncaws ua haujlwm zoo. Ib xyoo warranty, pub dawb online pabcuam rau cov neeg siv khoom txawv teb chaws.

Cim npe nrov: Earthworm Freeze Dryer, Tuam Tshoj, Hoobkas, lwm tus neeg, manufacturers, customized, nqe, ua nyob rau hauv Suav teb